My Homeschool Science Experiment: Testing a leaf for starch ( 2nd and 3rd attempt )

This is the second attempt:

We did the steps like the 1st attempt, only, we get different results.

This time we use a smaller and weaker leaf, and it looks like the chlorophyll from the leaves are released quite fast! The ethanol is quickly green in a few minutes, not like the tanjung leaf.
The result:

I think we have boiled the leaf too long, not only ridding off the membrane, but also boiling out the startch. The betadine shows that none of thoe two leaves have startch, Or maybe the leaves doesn't have any startch at all. The leaf at the left is as a fresh leaf as a control.

The third attempt:

At the left, it is the vegetable from the refrigerator for a few days. While the leaf at the right is freshly picked from a plant.

These leaves are boiled in a shorter time than the 2nd attempt.
I put those leaves into the test tube with ethanol, and the chlorophyll drained out quicker. It's so green in just a few minutes.

See the green? It is the greenest among all three trials. That means we broke the membrane really well.
 





 The freshly picked leaf contains startch, as the betadine turns black, while the leaf from the fridge contains very little startch.